Procedure for Isotachophoretic Determination of Thiodiglycolic Acid in Urine Using Desalination of the Analyzed Sample

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  • J. Chylkova J. Heyrovsky Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, Prague
  • R. Fadrna J. Heyrovsky Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, Prague

Abstrakt

A procedure for the isotachophoretic determination of thiodiglycolic acid (TDGA) in urine after desalination and pH adjustment of analyzed samples was elaborated. The salts present in urine were precipitated by addition of ethanol. TDGA was determined in anionic isotachophoretic mode in 0.01 M HCl as a leading electrolyte containing 0.01 % (2-hydroxyethyl)cellulose at pH 4.0 (adjusted with 6-aminohexanoic acid). 0.01 M acetic acid was used as a terminating electrolyte. The reproducibility of TDGA determination was very good, the relative standard deviation was less than 1.5 % for a concentration of TDGA 90 mg.l-1.

Publikováno

15.06.2004

Jak citovat

Chylkova, J., & Fadrna, R. (2004). Procedure for Isotachophoretic Determination of Thiodiglycolic Acid in Urine Using Desalination of the Analyzed Sample. Chemické Listy, 98(5). Získáno z http://chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/2140

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