Immunochemical Analysis of von Willebrand Factor and Its Clinical Use

Abstract

The aim of the present work is the laboratory confirmation (or exclusion) of the von Willebrand disease (VWD) diagnosis in patients with a suspect bleeding disorder and determination of the disease type. We used electrophoretic separation followed by an immunochemical chemiluminescent detection of the von Willebrand factor multimers. In the tested sample of 260 patients (in 2000–2010), all the three primary categories of the VWD were detected. The VWD categories were of type 1 (71 %), of type 2A/B/E (24.7 %) and of type 3 (3.6 %). Our findings in the Czech population are close to the data of other research groups.