Chronopotentiometric Stripping Analysis of Proteins and Their Interactions

Dedicated to Professor Jiří Barek on the occasion of his 75ᵗʰ birthday

Authors

  • Tatiana Staroňová Institute of Biophysics, Czech Academy of Sciences, Brno, Czech Republic
  • Veronika Ostatná Institute of Biophysics, Czech Academy of Sciences, Brno, Czech Republic

DOI:

https://doi.org/10.54779/chl20240515

Keywords:

chronopotentiometric stripping analysis, protein interactions, peak H, electrochemical protein analysis, mercury electrode

Abstract

Chronopotentiometric constant current stripping analysis (CPS) is a highly sensitive method for protein analysis that does not require modification or labeling. During the CPS analysis, proteins yield the so-called peak H, resulting from the catalytic hydrogen evolution reaction. This peak is sensitive to both local and global changes in protein structure, allowing the study of individual proteins, as well as their complexes. CPS analysis has been utilized in studying biomedically important proteins involved in neurodegenerative diseases and cancer. In this article, we describe the development of CPS protein analysis and its progress over the past decade. We also demonstrate the versatility of the method and its potential applications.

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Published

2024-10-15

How to Cite

Staroňová, T., & Ostatná, V. (2024). Chronopotentiometric Stripping Analysis of Proteins and Their Interactions: Dedicated to Professor Jiří Barek on the occasion of his 75ᵗʰ birthday. Chemické Listy, 118(10), 515–519. https://doi.org/10.54779/chl20240515

Issue

Vol. 118 No. 10 (2024)

Section

Articles