Methods of Protein Digestion Utilizable in Proteomic Analysis

Authors

  • R. Čmelík Institute of Analytical Chemistry of the CAS, v.v.i., Brno
  • J. Bobálová Institute of Analytical Chemistry of the CAS, v.v.i., Brno

Keywords:

protein, enzymatic digestion, method, proteomics, enzyme

Abstract

Enzymatic digestion of analyzed proteins represents an important step of mass spectrometry-based proteomics. The present review summarizes recent alternatives to the standard protocol developed for efficient and fast protein digestion, such as application of organic solvents, microwaves, infrared radiation, ultrasound, and enzyme immobilization.

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Published

2016-03-15

How to Cite

Čmelík, R., & Bobálová, J. (2016). Methods of Protein Digestion Utilizable in Proteomic Analysis. Chemické Listy, 110(3), 174–178. Retrieved from http://chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/212

Issue

Vol. 110 No. 3 (2016)

Section

Articles