Cholinesterase Biosensor for Detection of Nerve Agents

Authors

  • K. Vymazalová Military Research Institute, State Enterprise, Brno | University of Defence, Faculty of Economics and Management, NBC Defence Institute, Vyškov
  • E. Halámek University of Defence, Faculty of Economics and Management, NBC Defence Institute, Vyškov
  • J. Kadlčák Military Research Institute, State Enterprise, Brno

Keywords:

detection, nerve agents, biosensor, cholinesterase reaction

Abstract

This article deals with a detection of nerve agents. A photocolorimetric biosensor was designed for the detection of this group of cholinesterase inhibitors. This biosensor utilizes the enzyme butyrylcholinesterase. Ellman's modification of the cholinesterase reaction was used for the determination. To test the detection ability of the proposed cholinesterase detection means, tabun, sarin, soman, cyclosarin, agent VX and R33 were used. The detector was able to detect these substances in the air at very low concentrations (5.79·10–8 – 4.33·10–7 mg L–1, depending on the type of inhibitor).

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Published

2016-09-15

How to Cite

Vymazalová, K., Halámek, E., & Kadlčák, J. (2016). Cholinesterase Biosensor for Detection of Nerve Agents. Chemické Listy, 110(9), 645–650. Retrieved from http://chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/166

Issue

Vol. 110 No. 9 (2016)

Section

Articles