Optimization of HPLC Determination of Clotrimazole

Abstract

A HPLC method for the determination of clotrimazole and its degradation products. (2-chlorophenyl)diphenylmethanol and imidazole, in Clotrimazol spray 1 % was developed. The development was focused on the selection of an optimal column, composition and pH of the mobile phase, and internal standard. A Discovery ZR-PBD column (5 ?m, 150 -× 4.6 mm) and spectrophotometric detection at 210 nm were selected. The optimal mobile phase was aqueous acetonitrile 1:1 (pH 9.7). The optimal injection volume and flow-rate were 3 ?l and 1 ml min^-1, respectively. On the basis of robustness tests, ibuprofen was used as the internal standard. The analysis time was less than 4.5 min.