Determination of Silybin in Blood Plasma Using High Performance Liquid Chromatography with Solid Phase Extraction

Autoři

  • P. Kosina Centre for Bioanalytical Research, Palacky University,
  • J. Bartek Institute of Medical Chemistry and Biochemistry, Medical Faculty , Palacky University, Olomouc

Abstrakt

HPLC method with SPE preseparation step for determination of silybin, the main constituent of silymarin, in blood plasma was optimized. A C18 sorbent for preseparation from acidified samples was used. Diastereomers of silybin in unconjugated form or total silybin are resolved by the method, the detection limit being 2 nmol/l and the limit of quantification 5 nmol/l per diastereomer. The method is suitable for silybin monitoring in human plasma after administration of drugs or food additives based on silymarin.

Stahování

Publikováno

15.01.2001

Jak citovat

Kosina, P., & Bartek, J. (2001). Determination of Silybin in Blood Plasma Using High Performance Liquid Chromatography with Solid Phase Extraction. Chemické Listy, 94(12). Získáno z http://chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/2464

Číslo

Vol 94 No 12 (2000)

Sekce

Články