Determination of Ionophoric Polyether Monocarboxylic Acids in Feedstuffs by HPLC with Post-Column Derivatization

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  • M. Douša Central Institute for Supervising and Testing in Agriculture Brno, Regional Laboratory, Plzeň

Abstrakt

A fast HPLC method for determination of low contents of monensin, salinomycin and narasin in feedstuff was developed and validated. The ionophores were extracted from samples with a hexane - ethyl acetate mixture and, after purification on a Sep-Pak Silica column, they were determined by HPLC on C18 reverse phase using post-column derivatization with 4-(dimethylamino)benzaldehyde and UV detection at 598 nm. The preseparation, separation and derivatization reaction of the ionophores were optimized. New derivatization reagents (salicylaldehyde, thiophene-2-carbaldehyde, 4-methoxybenzaldehyde, 4-hydroxy-3,5-dimethoxybenzaldehyde and ethylvanilin) were tested. For monensin, salinomycin and narasin, the respective parameters were as follows: determination limit 7.5, 266 and 503 μg.kg-1; repeatability 0.08 and 0.06 mg.kg-1 (content 0.2-5 mg.kg-1); recovery 99.0 ± 8.4, 97.0 ± 6.0 and 99.2 ± 5.1 % (content 1-5 mg.kg-1)

Publikováno

15.02.2005

Jak citovat

Douša, M. (2005). Determination of Ionophoric Polyether Monocarboxylic Acids in Feedstuffs by HPLC with Post-Column Derivatization. Chemické Listy, 99(1). Získáno z http://chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/2082

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