Stanovení 4-hydroxymethamfetaminu, 4-hydroxyamfetaminu, amfetaminu a methamfetaminu pomocí HPLC s fluorescenční detekcí
Klíčová slova:
methamfetamin, fluorescenční detekce, dansyl chlorid, HPLC, SPEAbstrakt
A rapid, sensitive and low-cost method for the determination of methamphetamine and its metabolites in urine was developed. The tested substances were isolated by SPE, dansylated and analyzed by HPLC with fluorescence detection. The method was validated, the limit of detection for methamphetamine was lower than 1 ng ml–1; the limit of quantitation was 3 ng ml–1.