Determination of Phenolic Substances in Plant Materials by Capillary Electrophoresis and Liquid Chromatography
AbstraktA simple, sensitive and fast capillary electrophoretic method was optimized for the identification and determination of allelopathic phenolic compounds (vanillin, caffeic, 4-hydroxybenzoic, ferulic, salicylic, protocatechuic and 4-coumaric acids) in plant materials. Separation in an uncoated silica capillary (Supelco Select - FS75, effective length 22 cm, total length 31 cm, 75 mm I.D., 363 mm O.D.) was performed at 6 kV in 20 mmol/l boric acid and 60 mmol/l Na2HPO4 buffer (pH 8.75). The method was applied to the determination of vanillin, 4-hydroxybenzoic and protocatechuic acids in Ranunculus bulbosus, Veronica chamaedrys and Sanguisorba minor and in barley seeds. The results were in good agreement with those obtained by liquid chromatography.
Vlcek, J., Klejdus, B., & Kuban, V. (2002). Determination of Phenolic Substances in Plant Materials by Capillary Electrophoresis and Liquid Chromatography. Chemické Listy, 96(1). Získáno z http://chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/2357