Optimization of HPLC Determination of Clotrimazole

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  • P. Žáková Department of Analytical Chemistry, Faculty of Pharmacy, Charles University, Hradec Králové
  • H. Sklenářová Department of Analytical Chemistry, Faculty of Pharmacy, Charles University, Hradec Králové
  • L. Havlíková Department of Analytical Chemistry, Faculty of Pharmacy, Charles University, Hradec Králové
  • L. Matysová Department of Analytical Chemistry, Faculty of Pharmacy, Charles University, Hradec Králové
  • D. Šatínský Department of Analytical Chemistry, Faculty of Pharmacy, Charles University, Hradec Králové

Abstrakt

A HPLC method for the determination of clotrimazole and its degradation products. (2-chlorophenyl)diphenylmethanol and imidazole, in Clotrimazol spray 1 % was developed. The development was focused on the selection of an optimal column, composition and pH of the mobile phase, and internal standard. A Discovery ZR-PBD column (5 ?m, 150 -× 4.6 mm) and spectrophotometric detection at 210 nm were selected. The optimal mobile phase was aqueous acetonitrile 1:1 (pH 9.7). The optimal injection volume and flow-rate were 3 ?l and 1 ml min-1, respectively. On the basis of robustness tests, ibuprofen was used as the internal standard. The analysis time was less than 4.5 min.

Publikováno

15.04.2009

Jak citovat

Žáková, P., Sklenářová, H., Havlíková, L., Matysová, L., & Šatínský, D. (2009). Optimization of HPLC Determination of Clotrimazole. Chemické Listy, 103(3). Získáno z http://chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/1567

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