Utilizing Automated Chip Electrophoresis for Study of Lactoferrin and Matrix Metalloproteinases

  • O. Zítka Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University, Brno,
  • S. Křížková Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University, Brno,
  • V. Adam Department of Animal Nutrition and Forage Production, Faculty of Agronomy, Mendel University, Brno,
  • A. Horna Radanal Ltd., Pardubice,
  • J. Kukačka Department of Clinical Biochemistry and Pathobiochemistry, 2nd Faculty of Medicine, Charles University, Prague,
  • R. Průša Department of Clinical Biochemistry and Pathobiochemistry, 2nd Faculty of Medicine, Charles University, Prague,
  • V. Žížková Research Institute for Knitting, Brno
  • R. Kizek Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University, Brno,

Abstrakt

Automated chip capillary electrophoresis was used in detection and isolation of lactoferrin and a human matrix metalloproteinase (MMP-9) as well as in investigation of interactions and cleavage of collagen with MMP-9. The method is a sensitive and simple technique superior to SDS-PAGE. It is useful in proteomic research.
Publikované
2010-04-15
Sekce
Články