Study of Immobilized and Extracellular Dipeptidylpeptidase IV in Chelidonium maius

  • M. Koreňová Medicinal Plants Garden,
  • J. Stano Department of Cellular and Molecular Biology of Drugs, Faculty of Pharmacy, Comenius University, Bratislava, Slovakia,
  • K. Mičieta Department of Botany, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia,
  • A. Barth Institute of Biochemistry and Biotechnology, Martin Luther University,Halle/Saale, Germany,
  • P. Nemec Export-Import Ltd, Bratislava, Slovakia
  • V. Blanáriková Department of Cellular and Molecular Biology of Drugs, Faculty of Pharmacy, Comenius University, Bratislava, Slovakia,

Abstrakt

Cells in suspension culture of celandine (Chelidonium maius L.) were permeabilized with Tween 80 and immobilized with glutaraldehyde, alginate or pectinate. Glutaraldehyde-immobilized celandine cells lost their viability. Celandine cells immobilized with pectinate or alginate have retained the high activity of dipeptidylpeptidase (DP) IV/CD 26 with a pH optimum at 7.8. The immobilized cells showed a good activity, fair stability and convenient physicomechanical properties. The extracellular activity of DP IV/CD 26 estimated in cell suspension accounts for 82.2 % of the total activity the rest being due to the intracellular activity. The latter activity is 1.87 times higher than the extracellular one. Secretion of DP IV/CD 26 by root tips, root hairs, callus and suspension cells was detec¬ted histologically. The method permits a rapid, simple and specific identification of DP IV/CD 26.
Publikované
2012-10-15
Sekce
Články