LC-MS - an Objective Diagnostic Method of Amanita Intoxications

  • B. Merová Institute of Forensic Medicine, Faculty of Medicine, Palacky University, Olomouc,
  • M. Staňková Institute of Forensic Medicine, University Hospital Ostrava,
  • J. Stříbrný Institute of Forensic Medicine, Central Military Hospital, Prague
  • P. Ondra Institute of Forensic Medicine, Faculty of Medicine, Palacky University, Olomouc,

Abstrakt

A LC-MS method for determination of muscarine and amatoxins in urine and blood serum was developed and validated. The analysis was carried out using an LC/MS quadrupole device equipped with electrospray. The separation of muscarine, α-amanitin, β-amanitin, phalloidin and phallacidin was performed on analytical columns in the gradient mode. The applicability of the method has been demonstrated by analyzing clinical samples.
Publikované
2012-10-15
Sekce
Články