Stanovení nikotinové kyseliny a jejích metabolitů pomocí HPLC-MS/MS

  • K. Žantová Univerzita Karlova v Praze, 1. lékařská fakulta, 4. interní klinika, Praha
  • E. Tvrzická Univerzita Karlova v Praze, 1. lékařská fakulta, 4. interní klinika, Praha
  • M. Vecka Univerzita Karlova v Praze, 1. lékařská fakulta, 4. interní klinika, Praha
  • A. Žák Univerzita Karlova v Praze, 1. lékařská fakulta, 4. interní klinika, Praha
Klíčová slova: nikotinová kyselina, HPLC-MS, niacin, extrakce pevnou fází, metabolity niacinu

Abstrakt

Nicotinic acid is a hypolipidemic agent with pleiotropic effects.  Its drug form with controlled release leads to pyridine metabolites associated with the risk of hepatotoxicity. The aim of the present study was to introduce a HPLC-MS method for the determination of nicotinic acid, nicotinamide, nicotinuric acid, N-methylnicotin­amide, nicotinamide 1-oxide, 1-methyl-2-oxo-1,2-dihydro­pyridine-5-carboxamide, and 1-methyl-4-oxo-1,4-dihydro­pyridine-5-carboxamide in blood plasma or urine. The method is useful for the monitoring in hypolipidemic the­rapy. We have compared calibration  curves of individual metabolites using Hypercarb (graphite carbon) and Hypersil Silica (silicagel) columns. Both columns gave linear calibration curves for all the mentioned analytes (except for 1-methylnicotinamide) in the concentration range 10–2000 ng ml–1. The calibration curve in blood plasma and urine measured with the Hypersil Silica column was linear in the range 20–4000 ng ml–1 for all the tested analytes. The analysis time was 15 min with the column Hypercarb and 27 min with Hypersil Silica. Biological samples were extracted with sulfonated copolymer N-vinylpyrrolidone – divinylbenzene. Reproducibility of the results for biological samples varied between 0.06 % and 19.97 %.

Publikované
2015-12-15
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