32P-Postlabelling: A Sensitive Technique for the Detection of DNA Adducts

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  • M. Stiborova Department of Biochemistry, Faculty of Science, Charles University, Prague,
  • E. Frei Department of Molecular Toxicology, German Cancer Research Centre, Heidelberg, Germany
  • C. A. Bieler Department of Molecular Toxicology, German Cancer Research Centre, Heidelberg, Germany
  • H. H. Schmeiser Department of Molecular Toxicology, German Cancer Research Centre, Heidelberg, Germany

Abstrakt

The 32P-postlabelling assay has emerged as a major method to detect DNA adducts induced by structurally diverse carcinogens and other DNA lesions. The technique comprises enzymatic degradation of DNA to 3'-monodeoxynucleotides, enrichment of adducts, 5'-32P-labelling, adduct separation by TLC (or HPLC), and detection and quantitation of adducts. The review concerns individual 32P-postlabelling techniques (standard procedure, enrichment methods) as well as the applications of the method to different DNA modifications. Recent reports on the application of the 32P-postlabelling technique in human risk assessment using carcinogen-DNA adducts as molecular biomarkers are discussed and a critical evaluation of the assays is presented.

Publikováno

15.09.1998

Jak citovat

Stiborova, M., Frei, E., Bieler, C. A., & Schmeiser, H. H. (1998). 32P-Postlabelling: A Sensitive Technique for the Detection of DNA Adducts. Chemické Listy, 92(8). Získáno z http://chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/2681

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