Three-dimensional Fluorescence Spectra - A Resolvable Problem also with Old Instruments
a Department of Medicinal Chemistry and Biochemistry, Faculty of Medicine, Pavel Jozef Safarik University, Kosice, b Kurska St. 25, Kosice, Slovak Republic
The possibility of obtaining fluorescence spectra in the form of three-dimensional graphs after modification of an old spectral fluorimeter is described. A Perkin-Elmer model 3000 instrument was innovated to provide, through a hardware interface, the direct output of measured spectral quantities into a computer, where they are worked up, using a special program Spektro, in the shape of three-dimensional graphs or spectral contour maps. By this modification, the instruments achieve several parameters of the latest superior spectrofluorimeters.
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