Quantitative Chemiluminiscent Detection of Plant Peroxidases Using a Commercial Kit Originally Designed for Blotting Assays

Page: 130

M. Mackovaa, E.N. Ferrib, K. Demnerovaa, and T. Macekc

aDepartment of Biochemistry and Microbiology, Institute of Chemical Technology, Prague, bInstitute of Chemical Science, University of Bologna, Italy, cInstitute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague


The paper describes an improvement of chemiluminescent analysis of plant peroxidases by the use of a commercial kit originally designed for qualitative Western blotting assays. The chemiluminescent method using the reagents of the Amersham ECL kit offers a good alternative to the well known and widely exploited colorimetric or chemiluminescent methods using luminol. The proposed procedure significantly improves the chemiluminescent enzyme assay with labour-consuming separate mixing of all components by application of standard and ready-to-use solutions.


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