Covalent Chromatography

Page: 314

Z. Glatz

Department of Biochemistry, Faculty of Science, Masaryk University, Brno


Covalent chromatography is an important, highly specific separation technique. In covalent chromatography, proteins and other ligands bearing accessible thiol groups form a mixed disulfide with the gel. The proteins are thus covalently linked to the stationary phase from which they can be subsequently eluted by addition of a reducing agent. Basic principles of the method are given together with the methods for the synthesis of gels. In addition, the paper describes several special applications of covalent chromatography.


Full text (PDF)