New Techniques in Diagnostics bv Capillary Electrophoresis of Hereditarv Disorders of Purine and Pvrimidine Metabolism

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D. Friedeckya, T. Adamb,c, J. Sevcika,c, and P. Bartaka,c

aDepartment of Analytical Chemistry, Palacký University, Olomouc, bLaboratory of Hereditary Metabolic Disorders, Institute of Clinical Biochemistry, Faculty Hospital, Olomouc, cCentre of Analytical Chemistry of Molecular Structures, Palacky University, Olomouc


Purine and pyrimidine compounds were analyzed by capillary electrophoresis in urine of patients with inherited disorders purine and pyrimidine metabolism. Two separation of systems (alkaline-anionic and acid-cationic mode) have been compared. The use of a strongly acidic background electrolyte (pH 1.8) improves the selectivity and informative pithiness of the method. A combination of both anionic and cationic modes increases the reliability of diagnosis in hereditary disorders.


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