32P-Postlabelling: A Sensitive Technique for the Detection of DNA Adducts

Page: 661

M. Stiborovaa, E. Freib, C. A. Bielerb, and H. H. Schmeiserb

aDepartment of Biochemistry, Faculty of Science, Charles University, Prague, bDepartment of Molecular Toxicology, German Cancer Research Centre, Heidelberg, Germany


The 32P-postlabelling assay has emerged as a major method to detect DNA adducts induced by structurally diverse carcinogens and other DNA lesions. The technique comprises enzymatic degradation of DNA to 3'-monodeoxynucleotides, enrichment of adducts, 5'-32P-labelling, adduct separation by TLC (or HPLC), and detection and quantitation of adducts. The review concerns individual 32P-postlabelling techniques (standard procedure, enrichment methods) as well as the applications of the method to different DNA modifications. Recent reports on the application of the 32P-postlabelling technique in human risk assessment using carcinogen-DNA adducts as molecular biomarkers are discussed and a critical evaluation of the assays is presented.


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