Interaction of Genotoxic 2-Nitrofluorene and Its Metabolites with DNA In Vivo and Its Monitoring Using Electrochemical DNA Biosensors In Vitro

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Š. Skalováa,b, K. Stávkováa, A. Hájkováa, J. Bareka, J. Fischera, J. Wangc, and V. Vyskočila

a Charles University, Faculty of Science, Department of Analytical Chemistry, UNESCO Laboratory of Environmental Electrochemistry, Prague, b J. Heyrovský Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, Prague, c Department of Nanoengineering, University of California, San Diego, La Jolla, CA, USA

Keywords: nitrated polycyclic aromatic hydrocarbons, 2-nitrofluorene, DNA adducts, DNA damage detection, DNA biosensors, carbon electrodes, mercury electrodes

 

This review is focused on genotoxic 2-nitrofluorene, which is a marker for the presence of nitrated polycyclic aromatic hydrocarbons, and summarizes the current knowledge about the negative effects of 2-nitrofluorene and its metabolites on living organisms, especially on their DNA. These findings obtained via in vivo investigations are compared with information obtained using electrochemical DNA biosensors, which represent very promising in vitro alternative to the study of processes proceeding in living organisms during the interaction of their DNA with various xenobiotic compounds.

 

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