Microdialysis and Mass Spectroscopy as a Key for Monitoring of Metabolites in Immature Brain

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K. Vondrákováa,b,c, L. Uttla,c,d, H. Kubováb, G. Tsenova,b,d, and P. Kačere

a National Institute of Mental Health, Klecany; b Department of Evolution Epileptology, Pgysiological Institute, ASCR, Prague; c Department of Physiology, Faculty of Science, Charles University, Prague; d Department of Neuroscience, Biomedicine and Motion Science, Faculty of Medicine, University of Verona, Italy; e University of Chemistry and Technology, Prague, Czech Republic

Keywords: microdialysis, immature brain, HPLC-MS, aminoacids

 

The perinatal period of life is a critical moment for a proper development of CNS, however it is a risky period, which is associated with many pathologies. Hence, it is important to acquire knowledge and combine new methodologies for understanding the developmental aspects of physiology/pathophysiology of the brain. We have successfully developed and applied the method of monitoring a tissue metabolism in the immature brain. Using a combination of microdialysis technique and HPLC-MS basal levels of amino acids and other low molecular weight molecules in the hippocampus of immature rats (12-days-old) were mapped. Our results demonstrate the suitability of chosen parameters of sampling and analysis for the monitoring of tissue biochemistry and metabolomic mapping in immature brain. Moreover, a potential of the proposed method can found a place in clinical practice and for biomarkers monitoring of various CNS diseases.

 

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