Methods of Protein Digestion Utilizable in Proteomic Analysis

Page: 174

R. Čmelík and J. Bobálová

Institute of Analytical Chemistry of the CAS, v. v. i., Brno

Keywords: protein, enzymatic digestion, method, proteomics, enzyme

 

Enzymatic digestion of analyzed proteins represents an important step of mass spectrometry-based proteomics. The present review summarizes recent alternatives to the standard protocol developed for efficient and fast protein digestion, such as application of organic solvents, microwaves, infrared radiation, ultrasound, and enzyme immobilization.

 

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