Analysis of Nucleotides in Biological Material
a Centre of the Region Haná for Biotechnological and Agricultural Research, Central Laboratories and Research Support, Faculty of Science, Palacký University, Olomouc, b Centre of the Region Haná for Biotechnological and Agricultural Research, Department of Genetic Resources for Vegetables, Medicinal and Special Plants, Crop Research Institute, Olomouc
Keywords: nucleotides, solid phase extraction, liquid chromatography, mass spectrometry, capillary electrophoresis
Nucleotides are essential constituents of all living organisms. Monitoring of their levels could provide valuable information about the energy status of native and/or cultivated cells. Moreover, the enzymes involved in nucleotide metabolism are often the targets of antiviral, anticancer and immunosuppressive drugs. This review brings an overview of methods used for analysis of endogenous nucleotides and their synthetic analogs in biological material of various origin. Advantages, disadvantages as well as the principles of the respective methods are briefly discussed.
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