Utilization of Direct Amplification Methodology for Plant Genome Analysis

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J. Žiarovská a, A. Hricová b, Z. Gálová c, M. Záhorský b, and D. Bošeľová a

aDepartment of Genetics and Plant Breeding, Slovak University of Agriculture in Nitra, bInstitute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, cDepartment of biochemistry and biotechnology, Slovak University of Agriculture in Nitra

Keywords: DNA amplification, direct PCR, iPBS markers, ivy, amaranth


In this paper, the first description of the application of direct PCR (polymerase chain reaction) on multilocus markers amplification is reported. Two plant species – Hedera helix, L. and Amaranthus cruentus, L. were used in direct PCR for iPBS (inter Primer Binding Site) markers. For both of them, the amplification was obtained that was not only fully reproducible but also corresponded to the results obtained by the same markers in PCR when the standard DNA extraction method was used. In total, 18 different iPBS markers were tested. Direct PCR can thus be considered to be potentially applicable in the laboratory to all the standard procedures of genotyping and to help to improve time management there.


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