A Method of Analysis of Isoprostanes in Salmon Tissue

Page: 305

M. Bolechováa,b, J. Čáslavskýa, D. Mácováa, M. Vávrováa, and M. Taggartc

a Institute of Chemistry and Technology of Environmental Protection, Faculty of Chemistry, Technical University, Brno. b Central Inspectional and Testing Institute in Agriculture, Brno, Czech Republic, c Environmental Research Institute, North Highland College, Thurso, UK

Keywords: oxidative stress, mass spectrometry, liquid chromatography, isoprostanes

 

A sensitive and reliable method is proposed allowing quantification of isoprostanes in salmon tissue using ultra-HPLC coupled to tandem MS. Solid phase extraction is used for sample concentration and purification. Repeatability, linearity, limits of detection and quantification were assessed by validation.

 

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