Chromatographic Determination of Polyunsaturated Fatty Acids in Selected Animal Tissues
Department of Food Technology, Mendel University, Brno
Keywords: ?-linolenic acid, eicosapentaenoic acid, docosahexaenoic acid, lipids, gas chromatography
The objective of the study is to standardize GC determination of polyunsaturated fatty acids in animal tissues by lipid extraction with a hexane/propan-2-ol mixture and subsequent derivatization with CH3ONa/BF3. The limits of detection and recoveries for 18:2n-6, 20:4n-6, 18:3n-3, 20:5n-3, 22:5n-3 and 22:6n-3 fatty acids was in the range 0.320.42 mg/100 g, and 83105 %, respectively. The content of physiologically important icosapentaenoic acid (EPA) in chicken breast meat, thigh meat, quail eggs and cricket nymphs was 3, 10, 51 and 21 mg/100 g of fresh matter (P < 0.05). The EPA contents in livers of laboratory rats fed diets supplemented with palm oil, safflower oil and fish oil was 4, 3 and 342 mg/100 g, respectively (P < 0.05).
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