Immobilization of Cellulolytic Enzymes on Poly(ethylene terephthalate) and Polyacrylamide Carriers
a Institute of Food Science and Biotechnology, Technical University, Brno, b Institute of Chemistry, Slovak Academy of Sciences, Bratislava,c Institute of Biotechnology and Food Science, Slovak Technical University, Bratislava
Keywords: cellulolytic enzymes, immobilization, poly(ethylene terephthalate), Sorsilen, Eupergit C
A cellulolytic complex was immobilized on poly-(ethylene terephthalate) (PET) carrier Sorsilen, polyacrylamide carrier Eupergit C and a newly developed carrier from crushed PET bottles. The properties of immobilized enzymes such as pH and temperature optima, thermal stability, storage and operational stability, enzyme kinetics and the mode of action of immobilized enzymes were determined. The results showed that the carrier from crushed PET bottles and Eupergit C seem to be suitable for immobilization of this enzyme complex since both have relatively good operational, storage and thermal stability, and do not change the mode of action of the enzyme complex. The carrier from waste PET bottles is also inexpensive; so the immobilized preparation could be suitable for industrial use.
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