Effect of Mineral Oil on In Vitro Fertilization and Embryo Cultivation

Page: 841

P. Cupperová, J. Antalíková, M. Simon, J. Jankovičová, and K. Michalková

Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Ivanka pri Dunaji

Keywords: culture system, mineral oil, embryo


The present review deals with the harmful effects of mineral oils used as protective layers in fertilization in vitro. The results have shown the importance of the high quality and purity of the mineral oils used for the purpose. Contaminants reducing the oil quality include peroxides, Zn and Triton X-100. The review also highlights the deleterious effects of UV radiation on the oil which are usually less considered. Further the importance of storage and transportation for maintaining a high-quality oil is emphasized.


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