Validation of HPLC Determination of 1-Hydroxypyrene in Urine

Page: 55

I. Bajusová and Ľ. Legáth

Department of Occupational Medicine and Clinical Toxicology, Faculty of Mesicine, P. J. Šafárik University, Košice and L. Pasteur University Hospital, Košice, Slovakia

Keywords: polycyclic aromatic hydrocarbons, 1-hydroxypyrene, HPLC, urine


Exposure to polycyclic aromatic hydrocarbons (PAH) is a serious hazard for human health. 1-Hydroxypyrene (1 HOP), a major metabolite of pyrene, is used as a bio¬marker for the assessment of an exposure to PAH. The article deals with the validation of the HPLC determination of 1-HOP with fluorescence detection. 1-HOP was isolated from urine by solid phase extraction. The detection and quantification limits are 0.08 and 0.26 ??????g l–1, respectively. RSD of precision and accuracy are lower than 9.8 %. The recoveries are 78.8–93.5 %. This method can be used to determine and monitor the urinary 1-HOP in workers exposed to PAH.


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