Preparation of Base-Modified DNA by Enzymatic Incorporation of Modified Nucleoside Triphosphates

Page: 320

M. Hoceka,b

a Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague, b Department of Organic Chemistry, Faculty of Science, Charles University, Prague


Synthesis of base-modified oligonucleotides and DNA by polymerase incorporations of modified nucleoside triphosphates (dNTPs) is summarized. The cross-coupling synthesis of modified dNTPs, methods of their enzymatic incorporations, as well as their applications in bioanalysis and chemical biology are discussed.


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