Electrochemical Determination of Low-Molecular-Weight Antioxidants in Blood Serum

Page: 64

A. Kračmarováa and M. Pohankaa,b

a Centre of Advanced Studies, Faculty of Military Health Sciences, University of Defence, Hradec Králové, b Karel English College in Brno

 

The aim of the study was to establish and optimize an electrochemical method for the assay of low-molecular-weight antioxidants (LMWA). We used square-wave vol¬tammetry (at 0–1.1 V) on screen-printed sensors with C working electrodes. Optimum parameters were: potential step and amplitude 5 mV and frequency 1 Hz. Ascorbic acid, trolox, N-acetylcysteine and melatonin were used as standard antioxidants to test the linearity and sensitivity of the method. Limits of detection were 0.09 mmol l–1 for ascorbic acid, 0.04 mmol l–1 for melatonin, 0.03 mmol l–1 for trolox, and 0.07 mmol l–1 for N-acetylcysteine. 20 ??????l samples were used. The suitability of the method for the assay was confirmed by the standard ferric reducing ability of plasma test. Both methods correlated well (correlation coefficient 0.9778).

 

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