Voltammetric Determination of Trace Amounts of 2-Aminofluoren-9-one with Preconcentration of the Analyte by Solid-Phase Extraction

Page: 234

A. Hájková, V. Vyskočil, and J. Barek

Charles University in Prague, Faculty of Science, University Centre of Excellence UNCE “Supramolecular Chemistry”, Department of Analytical Chemistry, UNESCO Laboratory of Environmental Electrochemistry, Prague

 

Optimum conditions were found for the determination of nanomolar and subnanomolar concentrations of genotoxic 2-aminofluoren-9-one (2-AFN) by adsorptive stripping differential pulse voltammetry (AdSDPV) at a hanging mercury drop minielectrode (HMDmE) after preliminary separation and preconcentration by solid-phase extraction (SPE) in LiChrolut RP-18 E (500 mg) columns. The adsorbed analyte was eluted with acetone, the solution then evaporated to dryness, and the residue dissolved in acetate buffer (pH 4.0) (AcB). A sample was then measured by AdSDPV at the HMDmE, with the accumulation potential 100 mV (vs. Ag|AgCl in 1 mol l–1 KCl) and the accumulation time 120 s. Linear calibration curves were obtained for 2∙10–11 – 1∙10–9 mol l–1 2-AFN, with the limit of quantification (LQ) ? 4∙10–11 mol l–1. The method was verified by the determination of 2-AFN in model samples of drinking and river water at concentrations 4∙10–11 – 1∙10–9 and 2∙10–10 – 1∙10–9 mol l–1 (LQ ? 6∙10–11 and 4∙10–10 mol l–1, respectively).

 

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