Voltammetric Determination of Trace Amounts of 2-Aminofluoren-9-one with Preconcentration of the Analyte by Solid-Phase Extraction
Charles University in Prague, Faculty of Science, University Centre of Excellence UNCE “Supramolecular Chemistry”, Department of Analytical Chemistry, UNESCO Laboratory of Environmental Electrochemistry, Prague
Optimum conditions were found for the determination of nanomolar and subnanomolar concentrations of genotoxic 2-aminofluoren-9-one (2-AFN) by adsorptive stripping differential pulse voltammetry (AdSDPV) at a hanging mercury drop minielectrode (HMDmE) after preliminary separation and preconcentration by solid-phase extraction (SPE) in LiChrolut RP-18 E (500 mg) columns. The adsorbed analyte was eluted with acetone, the solution then evaporated to dryness, and the residue dissolved in acetate buffer (pH 4.0) (AcB). A sample was then measured by AdSDPV at the HMDmE, with the accumulation potential 100 mV (vs. Ag|AgCl in 1 mol l1 KCl) and the accumulation time 120 s. Linear calibration curves were obtained for 2∙1011 1∙109 mol l1 2-AFN, with the limit of quantification (LQ) ? 4∙1011 mol l1. The method was verified by the determination of 2-AFN in model samples of drinking and river water at concentrations 4∙1011 1∙109 and 2∙1010 1∙109 mol l1 (LQ ? 6∙1011 and 4∙1010 mol l1, respectively).
Full text (PDF)