A Study of The Molten Globule State of Cytochrome c by Visco¬metry
a Department of Medicinal and Clinical Biochemistry and Labmed, Co., Faculty of Medicine, Košice, b Department of Biochemistry, Faculty of Natural Science, P. J. Šafárik University, Košice, c Depatment of Biophysics, Institute of Experimental Phy¬sics of Slovak Acadamy of Sciences, Košice
Combination of viscometry and CD spectrometry made it possible to suggest the existence of a new conformational state of cytochrome c at high temperatures and ionic strengths. The molten globule state of cytochrome c was found at acidic pH in the presence of high concentrations of a salt at 20 °C. A low-cooperative conformational change of the cytochrome c to the unfolded state was observed with increasing temperature. Viscometry is a suitable tool for studies of similar processes in biomacromolecules.
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