Study of Immobilized and Extracellular Dipeptidylpeptidase IV in Chelidonium maius

Page: 847

M. Koreňováa , J. Stanob, K. Mičietac, A. Barthd, P. Nemece, and V. Blanárikováb

a Medicinal Plants Garden, b Department of Cellular and Molecular Biology of Drugs, Faculty of Pharmacy, Comenius University, Bratislava, Slovakia, c Department of Botany, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia, d Institute of Biochemistry and Biotechnology, Martin Luther University,Halle/Saale, Germany, eExport-Import Ltd, Bratislava, Slovakia

 

Cells in suspension culture of celandine (Chelidonium maius L.) were permeabilized with Tween 80 and immobilized with glutaraldehyde, alginate or pectinate. Glutaraldehyde-immobilized celandine cells lost their viability. Celandine cells immobilized with pectinate or alginate have retained the high activity of dipeptidylpeptidase (DP) IV/CD 26 with a pH optimum at 7.8. The immobilized cells showed a good activity, fair stability and convenient physicomechanical properties. The extracellular activity of DP IV/CD 26 estimated in cell suspension accounts for 82.2 % of the total activity the rest being due to the intracellular activity. The latter activity is 1.87 times higher than the extracellular one. Secretion of DP IV/CD 26 by root tips, root hairs, callus and suspension cells was detec¬ted histologically. The method permits a rapid, simple and specific identification of DP IV/CD 26.

 

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