LC-MS - an Objective Diagnostic Method of Amanita Intoxications

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B. Merováa, M. Staňkováa,b, J. Stříbrnýc, and P. Ondraa

a Institute of Forensic Medicine, Faculty of Medicine, Palacky University, Olomouc, b Institute of Forensic Medicine, University Hospital Ostrava, c Institute of Forensic Medicine, Central Military Hospital, Prague

 

A LC-MS method for determination of muscarine and amatoxins in urine and blood serum was developed and validated. The analysis was carried out using an LC/MS quadrupole device equipped with electrospray. The separation of muscarine, α-amanitin, β-amanitin, phalloidin and phallacidin was performed on analytical columns in the gradient mode. The applicability of the method has been demonstrated by analyzing clinical samples.

 

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