Animal Lipoxygenases Mechanism of Catalysis in Relation to Formation of Biologically Active Eicosanoids
Department of Cellular and Molecular Biology of Drugs, Faculty of Pharmacy, Comenius University, Bratislava, Slovak Republic
Lipoxygenases form a family of nonheme iron-containing enzymes involved in the lipid peroxidation processes. They participate, together with cyclooxy¬genases and cytochrome P-450, in the arachidonic acid metabolism, forming a group of cell signal molecules and secon¬dary messengers called eicosanoids. The lipoxygenase-catalyzed reactions are highly controlled processes media¬ted by steric shielding of substrate by bulky amino acid side chains in the reaction center. Equally important is the channeling of molecular oxygen and the anchoring of substrate in a substrate-binding cavity. From the viewpoint of development of new effective drugs, study of lipoxygena¬¬ses and bio¬logically active eicosanoids opens new horizons for affec-ting pathological processes.
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