Disintegration of Human Sperm and Characterization of Its Antigens
a Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague, b Department of Gynecology and Obstetrics, Faculty of Medicine, Charles University and Faculty Hospital, Plzeň
For treatment of female immunological infertility and of sensitivity to sperm it is necessary to characterize particular sperm antigens recognized by antibodies. For disintegration of sperm, sonication and chemical or related methods (detergents, benzalkonium chloride, urea, hypoosmotic shock) were used. The characterization was performed by SDS-electrophoresis followed by immublotting using IgG antibodies from 45 sera of infertile females. The serum of an eight-year-old girl was used as a negative control. The use of Triton X-100 seems to be the best way for preparation of sperm protein extract. The interactions of serum IgG antibodies with 68 and 123 kDa sperm proteins were shown to be the most frequently recognized.
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