Utilizing Automated Chip Electrophoresis for Study of Lactoferrin and Matrix Metalloproteinases

Page: 197

O. Zítkaa,b, S. Křížkováb, V. Adamb,c, A. Hornad,e, J. Kukačkaf, R. Průšaf, V. Žížkovág, and R. Kizekb

a Department of Biochemistry, Faculty of Science, Masaryk University, Brno, b Department of Chemistry and Biochemistry, and c Department of Animal Nutrition and Forage Production, Faculty of Agronomy, Mendel University, Brno, d University Institute, Tomas Bata University, Zlin, e Radanal Ltd., Pardubice, f Department of Clinical Biochemistry and Pathobiochemistry, 2nd Faculty of Medicine, Charles University, Prague, g Research Institute for Knitting, Brno


Automated chip capillary electrophoresis was used in detection and isolation of lactoferrin and a human matrix metalloproteinase (MMP-9) as well as in investigation of interactions and cleavage of collagen with MMP-9. The method is a sensitive and simple technique superior to SDS-PAGE. It is useful in proteomic research.


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