Using the Ellman Method for In Vivo Testing of Cholinesterase Activity

Page: 46

J. Žďárová-Karasová, K. Kuča, D. Jun, and J. Bajgar

Department of Toxicology, Faculty of Military Health Service, Defence University, Brno


The changes in cholinesterase activity in tissues were evaluated and compared with the reactivation potential of reactivators. As reactivators, (E)-1-(4-carbamoylpyridi¬nium-1-yl)-4-{4-[(hydroxyimino)methyl]pyridinium-1-yl}but-2-ene dibromide (K203) and common 1,3-bis{4-[(hydroxyimino)methyl]pyridinium-1-yl}-2-oxapropane dichloride (obidoxime) were used. The reactivation of both oximes was monitored in blood and blood plasma. The reactivation of tabun-inhibited acetylcholinesterase (AChE) was higher using the newly synthesized K203.


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