Protein Analysis Using Automated Experion Chip Electrophoresis and Its Comparison with SDS-PAGE

Page: 33

J. Bártaa, V. Bártováa, and V. Čurnb

a Department of Plant Production, Faculty of Agriculture, University of South Bohemia, České Budějovice, b Biotechnological Centre, Faculty of Agriculture, University of South Bohemia, České Budějovice, Czech Republic

 

Two methods recommended for analysis of denatured proteins, automated Experion chip electrophoresis and SDS-PAGE, are compared. The Experion method is a novel technique based on combination of the LabChip microfluidic separation technique (Caliper Life Sciences) and sensitive fluorescent detection. Both methods were compared in molecular weight (MW) determination of a protein standard mixture, resolution of protein pairs of near molecular weights and estimation of the abundance of a target protein in the mixture to be purified. Both the methods are appropriate for MW determination and in purification of proteins. The accuracy of the methods is approximately the same (ca. 8 %), but the Experion method shows better reproducibility (ca. 1.48 %) than SDS-PAGE (ca. 2.17 %).

 

Full text (PDF)