Development of Solid-Phase Extraction and HPLC/MS Methods for Deoxynivalenol Determination in Barley and Malt

Page: 679

A. Ježkováa, J. Karasováb, V. Dohnala,b,c, and I. Polišenskád

a Department of Food Technology, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Brno, b Department of Toxicology, Faculty of Military Health Sciences, University of Defence, Hradec Králové, c Department of Chemistry, Faculty of Science, J.E. Purkyně University, Ústí nad Labem, d Agrotest Fyto Ltd, Kroměříž


A method for the determination of deoxynivalenol (DON) in cereals has been developed and tested. The method is based on solid phase extraction in a column and subsequent separation by HPLC-MS. DON was detected with a MS detector in positive mode. The method is inexpensive, fast and highly sensitive. The method was used for the determination of deoxynivalenol in barley and malt, with the detection limit 25-30 μg kg−1. Changes in the DON content after malting were followed using the method.


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