Determination of Lamotrigine by Isotachophoresis – Capillary Zone Electrophoresis

Page: 166

L. Budákováa,c, H. Brozmanováa, F. Kvasničkab, and M. Grundmanna,c

a Department of Clinical Pharmacology, University Hospital Ostrava and Faculty of Health Studies, University of Ostrava, Ostrava, b Department of Food Preservation and Meat Technology, Institute of Chemical Technology, Prague, c Department of Pharmacology, Faculty of Medicine, Palacký University, Olomouc

 

The aim of this work was to develop and validate electrophoretic methods for the determination of a new antiepileptic, lamotrigine (LAM). We tested the conditions for analysis of LAM in blood serum by two capillary isotachophoreses (ITP-ITP) and by capilary isotachophoresis – capillary zone electrophoresis (ITP-CZE). Best results were obtained using the ITP-CZE method with UV detection at 254 nm. The latter method offers a simple and rapid procedure for the determination of LAM in blood serum. A low sample volume is highly convenient especially for the determination in children. The analysis takes some 20 min. The linearity of the method was proved in the range 0?20 mg LAM/l. The limits of detection and quantification were 0.25 and 0.83 mg LAM/l, respectively. The variation coefficients were below 10 % at three concentration levels (1, 7 and 20 mg l????????????1). The method was compared with HPLC using the Passing-Bablok regression analysis; no significant differences were found.

 

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