Comparison of Analytical Methods for Rutin Determination in Tartary Buckwheat
Crop Research Institute, Prague, Czech Republic
Rutin is a natural antioxidant useful for human health. The aim of this study was to compare some analytical methods for rutin determination in a plant material ? tartary buckwheat (Fagopyrum tataricum (L.) Gaertn.) - of different origin. The methods used were HPLC/UV and two spectrophotometries. Both spectrophotometric methods gave higher values than HPLC/UV. The results mutually correlated well, except those obtained by the spectrophotometric method using AlCl3. The method proved unsuitable for the purpose. The other spectrophotometric method overestimated the rutin content in leaves and stems.
Full text (PDF)