The Effect of Simulated Digestion on Element Speciation in Rye Flakes
Institute of Chemical Technology, Technická 5, 166 28 Prague 6, Czech Republic
To investigate element speciation in digested rye flakes, a simulated digestion using pig’s pepsin and pancreatin was performed at 37 °C. The digest was analyzed by SEC/ICP-MS (Superdex 75, 0.02 mol l-1 Tris-HCl buffer). Most elements in flakes are solubilized by enzymatic hydrolysis. P and Mo recoveries in SEC analysis of the digest approach to 100 %, those of Fe and Cu range from 60 to 80 % and that of Zn varies from 10 to 40 %. Ionic species or labile complexes were not recovered. The proportions of elements change as a result of enzyme digestion. No Fe is recovered in SEC analysis of flake extracts. Most Fe in the digest is bonded to a medium-molecular-weight fraction. Low-molecular-weight (<2 kDa) fractions with bonded Cu and Zn partly persist in the digest.
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