Application of Flow Cytometry of Yeast in Research and Practice

Page: 183

J. Novák, G. Basařová, J. Fiala, and P. Dostálek

Department of Fermentation Chemistry and Bioengineering, Institute of Chemical Technology, Prague

 

Flow cytometry as a rapid technique can be used for monitoring the yeast (Saccharomyces cerevisiae) growth in biotechnological processes. The technique permits analysis of single cells in suspension and thus to obtain cell size distribution, granularity, intracellular contents of DNA, proteins, and sterols, viability, cell cycle, intracellular pH and enzyme activities. Flow cytometry can also be used for cell sorting. Despite the advantages of flow cytometry, implementation of the technique in biotechnological laboratories is still limited.

 

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