Application of the Brdička Reaction in Determination of Metallothionein in Patients with Tumours

Page: 51

V. Adama, O. Blaštíka, S. Křížkováa, P. Lubalb, J. Kukačkac, R. Průšac, and R. Kizeka

a Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Brno, b Department of Analytical Chemistry, Masaryk University, Brno, c Department of Clinical Biochemistry and Pathobiochemistry, Charles University, Prague


The connection between the metallothionein (MT) content and the presence of tumor was studied by adsorptive transfer stripping technique and differential pulse voltammetry (Brdička reaction). The average content of MT in the blood serum of tumour patients was higher than in controls (0.72 ± 0.09 μmol dm−3). In leukemia and lung cancer, the MT content was more than five times higher in comparison with control.


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