The Study of Induced Circular Dichroism in the Porphyrin-Poly(L-glutamic acid) Complexes

Page: 246

O. Julíneka and M. Urbanováb

a Department of Analytical Chemistry and b Department of Physics and Measurements, Institute of Chemical Technology, Prague, Czech Republic


The formation conditions for the binary complexes composed of poly(glutamic acid) and cationic porphyrins 5,10,15,20-tetrakis{4-[(trimethylammonio)methyl]phenyl}porphyrin tetrachloride (TATP), 5,10,15,20-tetrakis(1-methyl¬pyridinum-4-yl)porphyrin (TMPyP) and its Cu(II) derivative (CuTMPyP), and the ternary complexes composed of poly(glutamic acid), the same cationic porphyrins TATP, TMPyP and CuTMPyP and anionic porphyrin 5,10,15,20-tetrakis(4-sulfonatophenyl)porphyrin were studied by electronic circular dichroism spectroscopy. The binary chiral complexes are formed if any of the three cationic porphyrins is used and the polypeptidic matrix possesses the α-helical structure. The ternary chiral complexes are formed if the matrix exhibits α-helical structure and cationic porphyrins TMPyP or CuTMPyP are used. When the metal porphyrin is used, the intensity of circular dichroism bands in spectrum is one order higher.


Full text (PDF)