Chromatographic Analysis of Naphthoquinones in Plants

Page: 271

P. Babulaa, R. Mikelováb, V. Adamb, D. Potěšilb, J. Zehnálekb, R. Kizekb, L. Havelc, and Z. Sladkýa

aDepartment of Natural Drugs, University of Veterinary and Pharmaceutical Sciences, bDepartment of Chemistry and Biochemistry and cDepartment of Botany and Plant Physiology, Mendel University of Agriculture and Forestry, Brno


The aim of the work was to optimize the high performance liquid chromatography coupled with a diode array detector for the determination of naphthoquinones (1,4-naphthoquinone, lawsone; juglone and plumbagin). The optimal conditions were: mobile phase 0.1 mol l-1 acetic acid – methanol (35 : 65 v/v), flow rate 0.8 ml min-1, column and detector temperature 40 °C. Detection limits (3 S/N) were 65 ng ml-1, 50 ng ml-1, 75 ng ml-1 and 39 ng ml-1 for lawsone, 1,4-naph¬tho¬qui¬none, juglone and plumbagin, respectively. The optimized method was used for the determination of the mentioned naphthoquinones in plants (Dionaea muscipula, Drosera rotundifolia, Drosera spathulata, Drosera capensis and Paulownia tomentosa).


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